Review




Structured Review

Fisher Scientific humidified incubator for hybridization
Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Humidified Incubator For Hybridization, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+incubator/for+humidified+hybridization+incubator/pmc13011240-38-0-5
Average 86 stars, based on 1 article reviews
humidified incubator for hybridization - by Bioz Stars, 2026-09
86/100 stars

Images

1) Product Images from "Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH"

Article Title: Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH

Journal: STAR Protocols

doi: 10.1016/j.xpro.2026.104441

Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Figure Legend Snippet: Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.

Techniques Used: In Situ Hybridization, Staining

Related Articles

other:

Article Title: Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum
Article Snippet: Excess radioactive material was 559 removed with NucAway spin columns (Ambion), and membrane prehybridization and 560 hybridization with the radioactive probe was conducted with ultrasensitive hybridization buffer 561 (Ambion) and incubated for 16 to 22 hours at 42°C under gentle agitation in a hybridization 562 incubator (Fisher Scientific).

Membrane:

Article Title: Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum
Article Snippet: Single-stranded oligonucleotide DNA probes used for hybridization (listed in Table S2) were labeled with [γ- 32 P]ATP by use of a phosphatase-negative T4 polynucleotide kinase (Affymetrix). .. Excess radioactive material was removed by use of NucAway spin columns (Ambion), and membrane prehybridization and hybridization with the radioactive probe were conducted with ultrasensitive hybridization buffer (Ambion), with samples incubated for 16 to 22 h at 42°C under gentle agitation in a hybridization incubator (Fisher Scientific). ..

Article Title: Vectors and cells for preparing immunoprotective compositions derived from transgenic plants
Article Snippet: .. The membrane was blocked with 5% skim milk+PBS+0.1% Tween-20 overnight at 4° C. or for two hours at room temperature using slow rotation in a hybridization incubator (Fisher Scientific, Tustin, Calif., USA). .. The membrane was washed twice briefly before incubating for one hour at 37° C. with a 1 in 50,000 dilution of the primary antibody, mouse anti-HN Mab14F antiserum (Benchmark Biolabs) in 1% skim milk+PBS+0.1% Tween-20.

Hybridization:

Article Title: Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum
Article Snippet: Single-stranded oligonucleotide DNA probes used for hybridization (listed in Table S2) were labeled with [γ- 32 P]ATP by use of a phosphatase-negative T4 polynucleotide kinase (Affymetrix). .. Excess radioactive material was removed by use of NucAway spin columns (Ambion), and membrane prehybridization and hybridization with the radioactive probe were conducted with ultrasensitive hybridization buffer (Ambion), with samples incubated for 16 to 22 h at 42°C under gentle agitation in a hybridization incubator (Fisher Scientific). ..

Article Title: Isolation and genetic manipulation of the antibiotic down-regulatory gene, wblA ortholog for doxorubicin-producing Streptomyces strain improvement.
Article Snippet: Cross-genome comparative transcriptome analyses were previously conducted using the sequenced Streptomyces coelicolor genome microarrays to understand the genetic nature of doxorubicin (DXR) and daunorubicin (DNR) overproducing industrial mutant (OIM) of Streptomyces peucetius.. In this previous work, a whiB-like putative transcription factor (wblAsco) was identified as a global antibiotic down-regulator in S. coelicolor (Kang et al., J Bacteriol 189:4315-4319, 2007).. In this study, a total genomic DNA library of a DXR/DNR-overproducing S. peucetius OIM was constructed and screened using wblAsco as a probe, resulting in the isolation of a wblA ortholog (wblAspe) that had 95% amino acid identity to wblAsco.

Article Title: Global gene expression profiling of androgen disruption in Qurt strain medaka.
Article Snippet: A B I M A E L L E Ó N , † P I N G S H I W U , ‡ L I N D A C . H A L L , § M I C H A E L L . J O H N S O N , † , | A N D S W E E J . T E H * , † Aquatic Toxicology Program, School of Veterinary Medicine, and Center for Watershed Sciences, University of California, Davis, California 95616, Lehigh University, Bethlehem, Pennsylvania, and Lawrence Livermore National Laboratory, Livermore, California

Article Title: Genome-wide transcriptional responses to sulfite in Saccharomyces cerevisiae.
Article Snippet: Sulfite is widely used as a preservative in foods, beverages, and pharmaceuticals because it is toxic to many microorganisms (Taylor et al., 1986).. Sulfite is the most effective at low pH against Gram-negative bacteria, followed in order of effectiveness by Gram-positive bacteria, molds, and yeast (Ough, 1993).. Depletion of ATP has been found to be largely responsible for toxicity of sulfite in Saccharomyces cerevisiae (Schimz and Holzer, 1979; Hinze and Holzer, 1986; Maier et al., 1986; Prakash et al., 1986).

Article Title: Chronic Ethanol Consumption Causes Alterations in the Structural Integrity of Mitochondrial DNA in Aged Rats
Article Snippet: Gels were then soaked in 0.25N HCl for 15 minutes, denaturation solution (1.5 mol/L NaCl, 0.5N NaOH) for 30 minutes, and neutralization solution (100 mmol/L Tris-HCl [pH 8.0], 1.5 mol/L NaCl) for a further 30 minutes before being transferred overnight by upward capillary action in 10× SSC buffer (1.5 mol/L NaCl, 150 mmol/L trisodium citrate [pH 7.0]) onto Biodyne A Membranes (Gibco BRL, Grand Island, NY). mtDNA was cross-linked to the membranes by baking at 80°C for 2 hours and detected using the PCR product from the cytochrome b region (see Polymerase Chain Reaction), which was labeled with Fluor-12-dUTP using the Prime-It Fluor Fluorescence Labeling kit (Stratagene, La Jolla, CA). .. Membranes were prehybridized in QuickHyb (Stratagene) for 15 minutes at 68°C and hybridized with the fluorescent probe along with 1 mg sonicated salmon sperm DNA for 2 hours at 68°C in a hybridization incubator (Fisher Scientific, Pittsburgh, PA). ..

Article Title: Molecular characterization and hypoxia-induced upregulation of neuronal nitric oxide synthase in Atlantic croaker: Reversal by antioxidant and estrogen treatments.
Article Snippet: Abbreviations: nNOS, neuronal nitric oxide synthase; RO reactive nitrogen species; NO, nitric oxide; NOx, nitrates an antioxidant; O2•, superoxide radical; ONOO−, peroxynitrit receptor; GRP30, G protein coupled receptor 30; ATD, 1,4,6 cytokines; IL-1β, interleukin-1β; NEM, N-ethylmaleimide; S L-arginine methyl ester; RT-PCR, reverse-transcription po rapid amplification of cDNA ends; GSP, gene specific pri ORF, open reading frame; FMN, flavin mononucleotide; F NADPH, nicotinamide adenine dinucleotide phosphate; DO ⁎ Corresponding author.. Tel.. : +1 361 749 6798; fax: + E-mail address: rahman@utexas.edu (M.S.

Article Title: Vectors and cells for preparing immunoprotective compositions derived from transgenic plants
Article Snippet: .. The membrane was blocked with 5% skim milk+PBS+0.1% Tween-20 overnight at 4° C. or for two hours at room temperature using slow rotation in a hybridization incubator (Fisher Scientific, Tustin, Calif., USA). .. The membrane was washed twice briefly before incubating for one hour at 37° C. with a 1 in 50,000 dilution of the primary antibody, mouse anti-HN Mab14F antiserum (Benchmark Biolabs) in 1% skim milk+PBS+0.1% Tween-20.

Incubation:

Article Title: Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum
Article Snippet: Single-stranded oligonucleotide DNA probes used for hybridization (listed in Table S2) were labeled with [γ- 32 P]ATP by use of a phosphatase-negative T4 polynucleotide kinase (Affymetrix). .. Excess radioactive material was removed by use of NucAway spin columns (Ambion), and membrane prehybridization and hybridization with the radioactive probe were conducted with ultrasensitive hybridization buffer (Ambion), with samples incubated for 16 to 22 h at 42°C under gentle agitation in a hybridization incubator (Fisher Scientific). ..

Gentle:

Article Title: Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum
Article Snippet: Single-stranded oligonucleotide DNA probes used for hybridization (listed in Table S2) were labeled with [γ- 32 P]ATP by use of a phosphatase-negative T4 polynucleotide kinase (Affymetrix). .. Excess radioactive material was removed by use of NucAway spin columns (Ambion), and membrane prehybridization and hybridization with the radioactive probe were conducted with ultrasensitive hybridization buffer (Ambion), with samples incubated for 16 to 22 h at 42°C under gentle agitation in a hybridization incubator (Fisher Scientific). ..

Article Title: Isolation and genetic manipulation of the antibiotic down-regulatory gene, wblA ortholog for doxorubicin-producing Streptomyces strain improvement.
Article Snippet: Cross-genome comparative transcriptome analyses were previously conducted using the sequenced Streptomyces coelicolor genome microarrays to understand the genetic nature of doxorubicin (DXR) and daunorubicin (DNR) overproducing industrial mutant (OIM) of Streptomyces peucetius.. In this previous work, a whiB-like putative transcription factor (wblAsco) was identified as a global antibiotic down-regulator in S. coelicolor (Kang et al., J Bacteriol 189:4315-4319, 2007).. In this study, a total genomic DNA library of a DXR/DNR-overproducing S. peucetius OIM was constructed and screened using wblAsco as a probe, resulting in the isolation of a wblA ortholog (wblAspe) that had 95% amino acid identity to wblAsco.

Article Title: Global gene expression profiling of androgen disruption in Qurt strain medaka.
Article Snippet: A B I M A E L L E Ó N , † P I N G S H I W U , ‡ L I N D A C . H A L L , § M I C H A E L L . J O H N S O N , † , | A N D S W E E J . T E H * , † Aquatic Toxicology Program, School of Veterinary Medicine, and Center for Watershed Sciences, University of California, Davis, California 95616, Lehigh University, Bethlehem, Pennsylvania, and Lawrence Livermore National Laboratory, Livermore, California

Article Title: Genome-wide transcriptional responses to sulfite in Saccharomyces cerevisiae.
Article Snippet: Sulfite is widely used as a preservative in foods, beverages, and pharmaceuticals because it is toxic to many microorganisms (Taylor et al., 1986).. Sulfite is the most effective at low pH against Gram-negative bacteria, followed in order of effectiveness by Gram-positive bacteria, molds, and yeast (Ough, 1993).. Depletion of ATP has been found to be largely responsible for toxicity of sulfite in Saccharomyces cerevisiae (Schimz and Holzer, 1979; Hinze and Holzer, 1986; Maier et al., 1986; Prakash et al., 1986).

Sonication:

Article Title: Chronic Ethanol Consumption Causes Alterations in the Structural Integrity of Mitochondrial DNA in Aged Rats
Article Snippet: Gels were then soaked in 0.25N HCl for 15 minutes, denaturation solution (1.5 mol/L NaCl, 0.5N NaOH) for 30 minutes, and neutralization solution (100 mmol/L Tris-HCl [pH 8.0], 1.5 mol/L NaCl) for a further 30 minutes before being transferred overnight by upward capillary action in 10× SSC buffer (1.5 mol/L NaCl, 150 mmol/L trisodium citrate [pH 7.0]) onto Biodyne A Membranes (Gibco BRL, Grand Island, NY). mtDNA was cross-linked to the membranes by baking at 80°C for 2 hours and detected using the PCR product from the cytochrome b region (see Polymerase Chain Reaction), which was labeled with Fluor-12-dUTP using the Prime-It Fluor Fluorescence Labeling kit (Stratagene, La Jolla, CA). .. Membranes were prehybridized in QuickHyb (Stratagene) for 15 minutes at 68°C and hybridized with the fluorescent probe along with 1 mg sonicated salmon sperm DNA for 2 hours at 68°C in a hybridization incubator (Fisher Scientific, Pittsburgh, PA). ..

Agarose Gel Electrophoresis:

Article Title: Molecular characterization and hypoxia-induced upregulation of neuronal nitric oxide synthase in Atlantic croaker: Reversal by antioxidant and estrogen treatments.
Article Snippet: Abbreviations: nNOS, neuronal nitric oxide synthase; RO reactive nitrogen species; NO, nitric oxide; NOx, nitrates an antioxidant; O2•, superoxide radical; ONOO−, peroxynitrit receptor; GRP30, G protein coupled receptor 30; ATD, 1,4,6 cytokines; IL-1β, interleukin-1β; NEM, N-ethylmaleimide; S L-arginine methyl ester; RT-PCR, reverse-transcription po rapid amplification of cDNA ends; GSP, gene specific pri ORF, open reading frame; FMN, flavin mononucleotide; F NADPH, nicotinamide adenine dinucleotide phosphate; DO ⁎ Corresponding author.. Tel.. : +1 361 749 6798; fax: + E-mail address: rahman@utexas.edu (M.S.



Similar Products

86
Fisher Scientific humidified incubator for hybridization
Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Humidified Incubator For Hybridization, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+incubator/for+humidified+hybridization+incubator/pmc13011240-38-0-5
Average 86 stars, based on 1 article reviews
humidified incubator for hybridization - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Taitec Corp hybridization incubator
Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Hybridization Incubator, supplied by Taitec Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+incubator/hybridization+incubator/pmc13116936-82-5-13
Average 86 stars, based on 1 article reviews
hybridization incubator - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

95
Bio-Rad frame seal incubation chambers
Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Frame Seal Incubation Chambers, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+incubator/Frame-Seal+in+situ+PCR+and+Hybridization+Slide+Chambers/pm41563121-189-1-4
Average 95 stars, based on 1 article reviews
frame seal incubation chambers - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier

90
FINEPCR Co Ltd hybridization incubator combi-d24
Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Hybridization Incubator Combi D24, supplied by FINEPCR Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+incubator/hybridization+incubator+combi+d24/pm40408618-130-28-31
Average 90 stars, based on 1 article reviews
hybridization incubator combi-d24 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
FINEPCR Co Ltd combi-d24 hybridization incubator
Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Combi D24 Hybridization Incubator, supplied by FINEPCR Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+incubator/hybridization+incubator+combi+d24/pm39939775-653-11-14
Average 90 stars, based on 1 article reviews
combi-d24 hybridization incubator - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

86
Taitec Corp hybridization incubator hb 80
Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Hybridization Incubator Hb 80, supplied by Taitec Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridization+incubator/hybridization+incubator/pmc12494442-67-12-15
Average 86 stars, based on 1 article reviews
hybridization incubator hb 80 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.

Journal: STAR Protocols

Article Title: Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH

doi: 10.1016/j.xpro.2026.104441

Figure Lengend Snippet: Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.

Article Snippet: Humidified incubator for hybridization , Fisher Scientific , 15-015-2632.

Techniques: In Situ Hybridization, Staining